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Spherix Inc
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Image Search Results
15 )." width="100%" height="100%">
Journal: Pediatric Research
Article Title: Molecular regulation of lung maturation in near-term fetal sheep by maternal daily vitamin C treatment in late gestation
doi: 10.1038/s41390-021-01489-4
Figure Lengend Snippet: Evaluation of target genes regulating oxidative stress, hypoxia signaling, glucocorticoid signaling, fetal lung liquid movement (controlled by chloride, sodium, and water movement), surfactant maturation, and airway remodeling by quantitative real-time RT-PCR (all primer sequences and concentrations previously published
Article Snippet: The membranes underwent 3 × 5 minutes washes in TBS-T and were incubated with the primary antibody overnight at 4 °C (ENAC-β (1:1000, in 5% BSA in TBS-T, #PA5-77817, Invitrogen; 87 kDa band);
Techniques: Quantitative RT-PCR, Marker, Activity Assay
Journal: Pediatric Research
Article Title: Molecular regulation of lung maturation in near-term fetal sheep by maternal daily vitamin C treatment in late gestation
doi: 10.1038/s41390-021-01489-4
Figure Lengend Snippet: Data are presented as normalized protein expression in arbitrary units (AU) for ENAC-β ( a ; 87 kDa band), Na-K-ATPase α1 ( c ; 110 kDa band), Na-K-ATPase β1 ( e ; 50 kDa band), 11βHSD-2 ( g ; 44 kDa band), SOD-1 ( i ; 24 kDa band), and SP-B ( k ; 8 kDa band) in saline (blue bars) and vitamin C (orange bars) groups. Data were analyzed by the Student’s unpaired t test. P < 0.05 was considered statistically significant. Western blot images represent target protein (upper panel) and reference protein (lower panel) for lambs in saline (S) and vitamin C (VC) groups. ⊗ = Not included in the analysis for this study. Beta-actin (β-actin; b , d , h ; 42 kDa band), cytochrome oxidase IV (COXIV; e ; 17 kDa band), Ponceau S ( j ; Total protein), and β-tubulin (β-tubulin; l ; 55 kDa band) are obtained from the same gel.
Article Snippet: The membranes underwent 3 × 5 minutes washes in TBS-T and were incubated with the primary antibody overnight at 4 °C (ENAC-β (1:1000, in 5% BSA in TBS-T, #PA5-77817, Invitrogen; 87 kDa band);
Techniques: Expressing, Western Blot